Protein Names. Studies of cerebrovascular permeability indicate that the BBB may be circumvented by conjugating hydrophilic molecules to monoclonal antibodies raised against peptides expressed by BCECs, e.g. Applications: IHC-P. Reactivity: Human. Complex between transferrin and soluble transferrin receptor for MAbs 8B9 and 11D3. Therefore, it holds great potential in T cell redirecting therapies. Bispecific T-cell engager antibodies (BiTE) have been explored as a means to recruit cytolytic T cells to kill tumor cells. It is ubiquitously distributed on the cell surface of actively growing human cells. NB100-64979 recognizes native, soluble and denatured forms of murine CD71. Anti-transferrin Antibody (D-9) is a mouse monoclonal IgG 1 λ transferrin antibody, cited in 7 publications, provided at 200 µg/ml. A 78 kDa-monomeric glycoprotein, transferrin is an important chelator with a primary function of serum iron transportation (Huebers and Finch 1987).It has the capacity to reversibly bind two atoms of ferric iron (Fe 3+) with high affinity (10 22 M −1 at pH 7.4) (Aisen et al. - Find MSDS or SDS, a COA, data sheets and more information. MCF-7 cells were subjected to SDS PAGE followed by western blot with 10084-2-AP (Transferrin receptor/CD71 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. An antibody that recognizes both human and murine TfR is needed to predict the toxicity of antibody-based agents before clinical trials, there is no such antibody to date. Transferrin receptor 1 (TfR1) levels, which vary inversely with iron status,13 were measured as J Neurochem. We offer a wide range of validated Transferrin Receptor antibodies. Description TFRC Polyclonal Antibody. Guichen Li, Xianghong Sun, Xiaona Wan, and Dongming Wang. The TFRC-holotransferrin complex is internalized by receptor-mediated endocytosis (summary by Jabara et al., 2016). This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin Receptor Antibody (13-6800) in WB. Background/Target Information The transferrin receptor (TfR), which is highly enriched in BCECs and choroid plexus epithelial cells (CPECs), is one of the most widely studied for RMT delivery.12–14 Much of the existing data have demonstrated the use of TfR-mediated transcytosis to increase the brain penetration of antibody … Transferrin receptor (TfR) is an endocytic receptor and identified as tumor relative specific due to its overexpression on most tumor cells or tissues, and TfR binds and intakes of transferrin-iron complex. The transferring-iron import system consists of a TonB dependent transporter (TbpA), and a lipoprotein co-receptor (TbpB) and both proteins work together in the transport of iron across the membrane. TA504563AM. Transferrin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. In the It binds to the transferrin receptor 1 (TfR1). Provider. The cTfRMAb-ScFv fusion protein is formed by fusion of the variable region of the heavy chain (VH) of the rat 8D3 MAb against the mouse transferrin receptor (mTfR) (yellow) to the amino terminus of mouse IgG1 constant (C) region (green), and fusion of a single chain Fv (ScFv) antibody against the A amyloid peptide to the carboxyl terminus of the heavy chain C-region. Iron uptake from transferrin is reasonably well understood, and involves the binding of transferrin to the transferrin receptor, internalization of transferrin within an endocytic vesicle by receptor-mediated endocytosis, and the release of iron from the protein by a decrease in endosomal pH. P M Friden, L R Walus, G F Musso, M A Taylor, B Malfroy, R M Starzyk. Human - Transferrin receptor - Sandwich ELISA, HRP-labelled antibody - for Plasma-EDTA - RUO - Immunoassays. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul. Transferrin receptor 2 antibody: Target information. MAb 1C10 does not cross-react with mouse, rabbit, bovine transferrin and with human lactoferrin. 18 Transferrin is one of the proteins that crosses the BBB through a mechanism of receptor-mediated transcytosis, which is utilized in the delivery of JR-141 to the brain. Results. The transferrin receptor 1 (TfR1), also known as cluster of differentiation 71 (CD71), is a type II transmembrane glycoprotein that binds transferrin (Tf) and performs a critical role in cellular iron uptake through the interaction with iron-bound Tf. Synonym: Transferrin 1 TF Reactivity: Human Coat, ELISA, IHC, StM Host: Mouse Monoclonal TF-3001 unconjugated. References The immunoreactivity of the anti-DMT1 antibody and the position of the DMT1 immunoreactive band were validated by using tissues from Dmt1 knockout animals (data not shown). antibodies, apoptosis, monoclonal antibodies, t-lymphocytes, transferrin receptors, human t-lymphotropic virus 1, neoplasms, transferrin Introduction Adult T-cell leukemia/lymphoma (ATL) is an aggressive lymphoproliferative disorder associated with the human T-cell lymphotropic virus type 1 (HTLV-1). View only Transferrin Receptor Monoclonal Antibodies or Transferrin Receptor Polyclonal Antibodies. CD71 is a disulfide-linked homodimer of 95-kDa subunits. Yoshikawa T, Pardridge WM (1992) Biotin delivery to brain with a covalent conjugate of avidin and a monoclonal antibody to the transferrin receptor. Three groups of six athymic mice bearing a human melanoma were injected via tail vein with (a) 0.55 mg … Anti Transferrin Receptor Antibody, supplied by Abcam, used in various techniques. Transferrin plays a major role in providing iron as a source for haemoglobin synthesis. 2009;102:1251-1258 3. CD71 is a 170-180 kDa type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. Fab(ulous) Hindrance. Here we show that ACE2 knockout … Specifications. UniProt Code History. transferrin Antibody (D-9) is recommended for detection of transferrin of human origin by WB, IP, IF, IHC (P) and ELISA. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (solid line histogram). The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. However, no ACE2 inhibitors have shown definite beneficiaries for COVID-19 patients, applying the presence of another receptor for SARS-CoV-2 entry. anti-Transferrin Receptor Antibodies 350 Transferrin Receptor (p90, CD71) (TFRC) Antibodies from 23 manufacturers are available on www.antibodies-online.com. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of IV dosing of TXB2 when fused to human Fc domain (TXB2-hFc) at 25 nmol/kg (1.875 mg/kg) in mice resulted in rapid binding to brain capillaries with subsequent transport into the brain parenchyma and specific uptake … 46 Taetle R, Castagnola J, Mendelsohn J. Mechanisms of growth 22 Neckers LM, Trepel JB. The development of vectors for cell-specific gene delivery is a major goal of gene therapeutic strategies. This protein is involved in the uptake of transferrin-bound iron into cells by endocytosis, although its role is minor compared to transferrin receptor 1 Function. The TfR-mediated incorporation of JR-141 was confirmed by using human fibroblasts in vitro. Erpantech Laboratory. Transferrin receptor antibody was raised in mouse using human soluble transferrin receptor as the immunogen. The uptake of a potential drug vector (OX26, or anti—transferrin receptor antibody IgG2a) conjugated to polyethyleneglycol-coated liposomes by BCECs was studied using in situ perfusion in 18-day-old rats in which the uptake of OX26 is almost twice as high as in the adult rat. Abstract: The present invention pertains to a method for delivering a neuropharmaceutical or diagnostic agent across the blood brain barrier to the brain of a host. Skip to main content Hello, Sign in. Monoclonal antibody to transferrin receptor blocks transferrin binding and inhibits human tumor cell growth in vitro. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Brain penetration of large molecule drugs is severely limited by the largely impermeable blood-brain barrier (BBB). Transferrin Receptor antibody was raised in mouse using purified human soluble transferrin receptor as the immunogen. Transferrin receptor (TfR) is an attractive molecule for targeted therapy of cancer. CD71, also known as the transferrin receptor (TfR1), is a transmembrane glycoprotein whose primary function is to mediate cellular uptake of iron from a plasma glycoprotein, transferrin. Assay Information: 10-T66D can be used as the detection antibody in a matched pair immunoassay in conjunction with 10-T66C as the capture antibody We have recently described a cationic immunoliposome system directed by a lipid-tagged, single-chain antibody Fv fragment (scFv) against the human transferrin receptor (TfR) that shows promising efficacy for systemic p53 tumor suppressor gene therapy in a human breast cancer metastasis model. of intact human IDS and anti-human transferrin receptor (hTfR) antibody, has been developed by JCR Pharmaceuticals.18 Transferrin is one of the proteins that crosses the BBB through a mechanism of receptor-mediated transcytosis, which is utilized in the delivery of JR-141 to the brain. In this work, we pursued TfR antibodies as potential cancer therapeutics, characterizing human single-chain variable antibody fragments (scFv) specific for the human TfR isolated from a phage display library. The transferrin receptor (TfR) is highly expressed on the surface of rapidly proliferating tumor cells. For instance, certain monoclonal antibodies against the transferrin receptor curb proliferation of tumor cells in vitro and in vivo (Lesley and Schulte, 1985); (White et al., 1990). Download Citation. Mouse placenta lysates probed with Rabbit Anti-Transferrin receptor Polyclonal Antibody, Unconjugated (bs-0988R) at 1:300 overnight at 4˚C. duces a monoclonal antibody against the human transferrin re-ceptor. Transferrin Receptor Antibody for Neuropathic Mucopolysaccharidosis II: A Phase 1/2 Trial Torayuki Okuyama,1 Yoshikatsu Eto,2 Norio Sakai,3 Kohtaro Minami,4 Tatsuyoshi Yamamoto, 4Hiroyuki Sonoda, Mariko Yamaoka, 4Katsuhiko Tachibana, Tohru Hirato,4 and Yuji Sato 1National Centre for Child Health andDevelopment, Tokyo157-8535,Japan; 2Advanced Clinical Research Centre & Asian Lysosome … G1/221/12 mouse transferrin receptor antibody. A 78 kDa-monomeric glycoprotein, transferrin is an important chelator with a primary function of serum iron transportation (Huebers and Finch 1987).It has the capacity to reversibly bind two atoms of ferric iron (Fe 3+) with high affinity (10 22 M −1 at pH 7.4) (Aisen et al. Host Mouse Type Primary Clonality Monoclonal (H68.4) Conjugate Unconjugated Target Transferrin receptor protein 1 UniProt P02786 - TFR1_HUMAN. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Transferrin receptor. The transferrin receptor (TfR) is highly expressed on the surface of rapidly proliferating tumor cells. Therefore, it holds great potential in T cell redirecting therapies. In this research, we developed a BiTE targeting TfR and CD3 (TfR-BiTE) and studied its therapeutic impact on TfR-positive cancer. The brain capillary endothelium, which makes up the blood-brain barrier (BBB) in vivo, expresses high concentrations of transferrin receptor, and recent studies show that an antitransferrin receptor monoclonal antibody may function as a BBB drug transport vector. - Find MSDS or SDS, a COA, data sheets and more information. HB21 was obtained from the American Type Culture Collection (14), propagated as ascites in BALB/c mice, and purified by precipitation at ammonium sulfate 50% saturation and affinity chromatography using Staphylococcus aureus Protein A. HB21 is of the lgG1 subclass. The present report examines the pharmacokinetics of clearance of radiolabeled antitransferrin receptor monoclonal antibody … This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. [125I]Mouse immunoglobulin G2a control antibody was cleared … The average level of expression by tumor is summarized in the table below. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Endotoxin level is <0.01EU/ug. Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor. Transferrin Receptor Antibody Fusion Protein for Neuronopathic Mucopolysaccharidosis II Hiroyuki Sonoda, 1,3 Hideto Morimoto, Eiji Yoden, 1Yuri Koshimura, Masafumi Kinoshita, Galina Golovina, Haruna Takagi, 1Ryuji Yamamoto, Kohtaro Minami, 1Akira Mizoguchi,2 Katsuhiko Tachibana, Tohru Hirato, and Kenichi Takahashi1,3 1Research Division, JCR Pharmaceuticals, Kobe, Japan; … Proceedings of the National Academy of Sciences Jun 1991, 88 (11) 4771-4775; DOI: 10.1073/pnas.88.11.4771. A BBB-penetrating fusion protein designated JR-141, which consists of intact human IDS and anti-human transferrin receptor (hTfR) antibody, has been developed by JCR Pharmaceuticals. Concentration: 1mg/ml. Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. Sonoda H. Abstract We report a case of anemia due to autoantibodies to the transferrin receptor interfering with iron incorporation by erythroid progenitors. These antibodies target Transferrin Receptor (CD71) in Human, Mouse, Rat, Non-human primate and Mustelid samples. Angiotensin-converting enzyme 2 (ACE2) has been suggested as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry to cause coronavirus disease 2019 (COVID-19). Transferrin receptor protein 1, p90, T9, TFR1_HUMAN. JR-141 has been shown to be distributed in the IHC Figures. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. Format: Liquid. Serum transferrin receptor (sTfR) is used as a means of ... detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients. For Research Use Only. Transferrin receptor (TfR) is an endocytic receptor and identified as tumor relative specific due to its overexpression on most tumor cells or tissues, and TfR binds and intakes of transferrin-iron complex. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity. Endosomal acidification leads to iron release. The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1-4). Monoclonal Antibody against the Human Transferrin Receptor (HB21). Based on the expertise of Upstate & Chemicon. Transferrin receptor 1 (TfR1) levels, which vary inversely with iron status,13 were measured as RRID: AB_2847210 We have previously generated an anti-TfR single-chain variable fragments of immunoglobulin (scFv) which were cloned from hybridoma cell line producing antibody against TfR … United States Patent 5182107 . Ligand for transferrin receptor is the serum iron transport protein, transferrin. Knockdown of Transferrin Receptor was achieved by transfecting HeLa cells with Transferrin Receptor specific validated siRNAs (Silencer® select Product # s725). 1697 Transferrin Receptor antibodies from 47 antibody suppliers Click each Transferrin Receptor antibody that interests you to view the full Transferrin Receptor antibody datasheet. Erythropoietin (EPO) is a potential therapeutic for Alzheimer’s disease (AD); however, limited blood–brain barrier (BBB) penetration reduces its applicability as a CNS therapeutic. Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Tell us about your platform and let our technical support help you in choosing the suitable products to your assay development project. Cancer Res. Results. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. 2010;70:5497-506 48. Anti-transferrin receptor (TfR)-based bispecific antibodies have shown promise for boosting antibody uptake in the brain. Various TfR-targeted therapeutic agents such as anti-TfR antibodies conjugated with anticancer agents have been developed. The TFRC gene encodes the transferrin receptor, which is important for cellular iron uptake. This is a Validated Antibody Database (VAD) review about human transferrin receptor, based on 526 published articles (read how Labome selects the articles), using transferrin receptor antibody in all methods.It is aimed to help Labome visitors find the most suited transferrin receptor antibody. Black: cells alone; Grey: isotype control; Green: AF488-labeled Transferrin Receptor antibody (TFRC/1059). Various TfR-targeted therapeutic agents such as anti-TfR antibodies conjugated with anticancer agents have been developed. This Transferrin Receptor antibody is available for research use only. However, TFR2 gene transcriptional regulation in hepatocytes remains largely unknown. When the Brain Shuttle (red/yellow) engages the transferrin receptor (blue), the Fab arms (green) of mAβ31 interfere with binding of the Fc stem (gray) to receptors on immune cells. The transferrin receptor is a glycoprotein composed of disulfide-linked polypeptide chains. Transferrin Receptor Monoclonal Antibody (DF1513) Catalog Number MA5-16644 Product Data Sheet Product Specific Information This product is a low-endotoxin, preservative-free formulation. Transferrin Receptor antibody Applications: WB, ELISA, IHC Reactivity: Mouse (Ms) Conjugate/Tag: Unconjugated Quantity: 1 ml IHC staining of human placenta using 10084-2-AP. CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. The IgG domain is a MAb against an endogenous BBB receptor such as the transferrin receptor (TfR). 21 matching Transferrin Receptor antibodies for Primate after applying filters Click each Transferrin Receptor antibody that interests you to view the full Transferrin Receptor antibody datasheet. Sol Ferrero Laboratory of Pharmaceutical Biotechnology, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina. Custom antibody services and bulk production also available. Nevertheless, there are limited data on the molecular properties, including affinity required for successful development of TfR-based therapeutics. CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. The transferrin receptor: role in health and Acad Sci USA 1981; 78: 3039–3043. Biotechnol Bioeng. Blockade of transferrin receptor function can halt cell division. Lesley J, Schulte R, Woods J. Modulation of transferrin receptor expression and function by anti-transferrin receptor antibodies and antibody fragments. However, antibodies against the transferrin receptor (TfR), enriched in brain endothelial cells, have been developed as delivery carriers of therapeutic cargoes into the brain via a receptor-mediated transcytosis pathway. Iron is required for multiple cellular processes and is essential for DNA synthesis and, thus, cellular proliferation. We have previously generated an anti-TfR single-chain variable fragments … This study describes the soluble production, purification, and functional testing of an anti-transferrin receptor single-chain antibody (OX26 scFv) using the yeast Saccharomyces cerevisiae. Engineering and expression of a chimeric transferrin receptor monoclonal antibody for blood-brain barrier delivery in the mouse. Bioz Stars score: 94/100, based on 1 PubMed citations. Useful in studies of dividing haematopoietic and tumor cell populations, and metabolic activity. Transferrin Receptor Antibody detects endogenous levels of total Transferrin Receptor. Targeting the transferrin receptor (TfR) on the surface of brain capillaries has been Exposure of some cell types to anti-PORIMIN (pro-oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Raised in: Rabbit. Transferrin receptor Polyclonal Antibody. Transferrin Receptor antibodies. Assay Information: 10-T119A can be used in a sandwich immunoassay as a conjugate antibody in conjunction with 10-T119C as the coating antibody See all options for Transferrin Receptor antibody. Immunocytochemistry - Anti-Transferrin Receptor antibody (ab84036) ab84036 staining Transferrin receptor protein 1 in HeLa cells. Observed Antibody Staining Data By Tissue Disease Status: Tissues from cancer patients, for instance, have their own distinct pattern of CD71 / Transferrin Receptor expression as measured by anti-CD71 / Transferrin Receptor antibody immunohistochemical staining. Transferrin Receptor antibody was raised in mouse using purified human soluble transferrin receptor as the immunogen. Account & Lists Returns & Orders.